Biology / Bio 1229 · Procedure · 60–90 seconds
Tracing One Replication Fork from Origin to Ligation
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This procedure traces one replication fork's machinery from the origin of replication through helicase, primase, both strands, and ligase to one continuous new molecule.
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The leading and lagging strands are synthesized by the same enzymes under the same 5'-to-3' rule — they differ in geometry, not in the chemistry of synthesis.