Biology / Bio 1217 · Atom · ~20 seconds
Helicase
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Helicase is the enzyme that unwinds DNA's double helix at a replication fork by breaking the hydrogen bonds between paired bases.
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Helicase uses energy from ATP hydrolysis to separate the two strands, exposing each as a single-stranded template.
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Helicase breaks hydrogen bonds between bases, not the covalent bonds of either strand's sugar-phosphate backbone.