Biology / Bio 0783 · Procedure · 60–90 seconds
The G-Protein Activation Cycle
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State
A heterotrimeric G-protein cycles between an inactive, GDP-bound state and an active, GTP-bound state, resetting itself once its own enzyme activity hydrolyzes GTP back to GDP.
Watch for
The G-protein resets itself by its own GTP hydrolysis — a signal will not terminate on schedule if that hydrolysis step is disabled.