Biology / Bio 0516 · Atom · ~20 seconds
The Active Site and Substrate Specificity
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An enzyme's active site is the region where its substrate binds, its amino acid arrangement shaped to fit one substrate or a narrow set.
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Each enzyme in a metabolic pathway binds only its own substrate, so glycolysis's ten steps require ten different enzymes.
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Specificity comes from the active site's precise shape and chemistry, not general stickiness to organic molecules.