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Biology  /  Bio 1529  ·  Atom · ~20 seconds

Southern and Northern Blotting

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Blotting transfers electrophoresis-separated fragments onto a membrane, then probes them with a labeled sequence; Southern blotting detects DNA, Northern detects RNA.

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A labeled probe binds only the membrane fragment complementary to it, revealing whether that sequence is present in the sample.

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Southern blots DNA and Northern blots RNA — Western blotting, a third technique, probes proteins instead of nucleic acids.

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