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Biology  /  Bio 1525  ·  Atom · ~20 seconds

PCR: Amplifying a Target DNA Sequence

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PCR amplifies one specific DNA region into many copies, using primers flanking the target and a heat-stable DNA polymerase.

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Two short primers bind opposite ends of the target sequence, and repeated heating and cooling cycles copy that stretch exponentially.

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PCR amplifies only the sequence its primers flank — it does not copy the whole genome indiscriminately.