Biology / Bio 1281 · Procedure · 60–90 seconds

Tracing Pre-mRNA Processing from Transcription to a Mature mRNA

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State

This procedure traces a eukaryotic pre-mRNA from RNA polymerase II's transcription through capping, tailing, and splicing to a finished, translatable mRNA.

Watch for

Capping, tailing, and splicing are three separate processing events, not stages of one single reaction — a pre-mRNA missing any one of the three is not yet a mature mRNA.

Builds on

1265Eukaryotic RNA Polymerases I, II, and III
1271The 5' Cap
1273The Poly-A Tail
1275Introns and Exons
1277Splicing and the Spliceosome
1279The 5' and 3' Untranslated Regions

Unlocks

1283Ribosome Structure: Large and Small Subunits
1295Tracing One Gene from Transcription Through a Finished Protein
1605Tracing a Trait from a Sequenced Gene to Proteome-Level Function